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3xnls mturquoise2  (Addgene inc)


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    Structured Review

    Addgene inc 3xnls mturquoise2
    FLIM images of HeLa cells co-transfected with <t>3xnls-Tq-Ca-FLITS</t> and G-Ca-FLITS-3xnes were acquired with a Leica Stellaris 8 before ( A ) and after addition of 100 µM histamine ( B ). False colors reflect the lifetime (average arrival time), according to the scale bar on the right. For the cyan channel, the excitation was at 440 nm and detection at 450–490nm and for the green channel, the excitation was at 490 nm and detection at 500–540nm.
    3xnls Mturquoise2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3xnls+mturquoise2/3xnls-mTurquoise2+(Plasmid+%2398817)/pmc12716836-297-10-11
    Average 93 stars, based on 5 article reviews
    3xnls mturquoise2 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "A green lifetime biosensor for calcium that remains bright over its full dynamic range"

    Article Title: A green lifetime biosensor for calcium that remains bright over its full dynamic range

    Journal: eLife

    doi: 10.7554/eLife.105086

    FLIM images of HeLa cells co-transfected with 3xnls-Tq-Ca-FLITS and G-Ca-FLITS-3xnes were acquired with a Leica Stellaris 8 before ( A ) and after addition of 100 µM histamine ( B ). False colors reflect the lifetime (average arrival time), according to the scale bar on the right. For the cyan channel, the excitation was at 440 nm and detection at 450–490nm and for the green channel, the excitation was at 490 nm and detection at 500–540nm.
    Figure Legend Snippet: FLIM images of HeLa cells co-transfected with 3xnls-Tq-Ca-FLITS and G-Ca-FLITS-3xnes were acquired with a Leica Stellaris 8 before ( A ) and after addition of 100 µM histamine ( B ). False colors reflect the lifetime (average arrival time), according to the scale bar on the right. For the cyan channel, the excitation was at 440 nm and detection at 450–490nm and for the green channel, the excitation was at 490 nm and detection at 500–540nm.

    Techniques Used: Transfection

    Related Articles

    Plasmid Preparation:

    Article Title: A green lifetime biosensor for calcium that remains bright over its full dynamic range
    Article Snippet: .. Of the following vectors the FP was exchanged for Tq-Ca-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag, 4xmts-mScarlet-I (Addgene plasmid #98818), ER-mScarletI (Addgene plasmid #137805) and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version. ..

    Article Title: A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium.
    Article Snippet: .. Of the following vectors the FP was exchanged for TqCa-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag, pLckmVenus-C1 (Addgene plasmid #84337) for a membrane tag, pmScarlet_Giantin_C1 (Addgene plasmid #85048) for a Golgi tag and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version. ..

    Article Title: A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium
    Article Snippet: .. Of the following vectors the FP was exchanged for Tq-Ca-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag, pLck-mVenus-C1 (Addgene plasmid #84337) for a membrane tag, pmScarlet_Giantin_C1 (Addgene plasmid #85048) for a Golgi tag and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version. ..

    Article Title: A green lifetime biosensor for calcium that remains bright over its full dynamic range
    Article Snippet: .. Of the following vectors, the FP was exchanged for Tq-Ca-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag, 4xmts-mScarlet-I (Addgene plasmid #98818), ER-mScarletI (Addgene plasmid #137805), and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version. .. The G-Ca-FLITS-3xnes (3x nuclear export sequence) was made by ligating it into 3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES (Addgene plasmid # 60492) that was cut with NdeI/BsrGI.

    Article Title: A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium
    Article Snippet: .. Of the following vectors the fluorescent protein was exchanged for Tq-Ca-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag, pLck-mVenus-C1 (Addgene plasmid #84337) for a membrane tag, pmScarlet_Giantin_C1 (Addgene plasmid #85048) for a Golgi tag and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version. ..

    Variant Assay:

    Article Title: A green lifetime biosensor for calcium that remains bright over its full dynamic range
    Article Snippet: .. Of the following vectors the FP was exchanged for Tq-Ca-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag, 4xmts-mScarlet-I (Addgene plasmid #98818), ER-mScarletI (Addgene plasmid #137805) and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version. ..

    Article Title: A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium.
    Article Snippet: .. Of the following vectors the FP was exchanged for TqCa-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag, pLckmVenus-C1 (Addgene plasmid #84337) for a membrane tag, pmScarlet_Giantin_C1 (Addgene plasmid #85048) for a Golgi tag and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version. ..

    Article Title: A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium
    Article Snippet: .. Of the following vectors the FP was exchanged for Tq-Ca-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag, pLck-mVenus-C1 (Addgene plasmid #84337) for a membrane tag, pmScarlet_Giantin_C1 (Addgene plasmid #85048) for a Golgi tag and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version. ..

    Article Title: A green lifetime biosensor for calcium that remains bright over its full dynamic range
    Article Snippet: .. Of the following vectors, the FP was exchanged for Tq-Ca-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag, 4xmts-mScarlet-I (Addgene plasmid #98818), ER-mScarletI (Addgene plasmid #137805), and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version. .. The G-Ca-FLITS-3xnes (3x nuclear export sequence) was made by ligating it into 3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES (Addgene plasmid # 60492) that was cut with NdeI/BsrGI.

    Article Title: A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium
    Article Snippet: .. Of the following vectors the fluorescent protein was exchanged for Tq-Ca-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag, pLck-mVenus-C1 (Addgene plasmid #84337) for a membrane tag, pmScarlet_Giantin_C1 (Addgene plasmid #85048) for a Golgi tag and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version. ..

    other:

    Article Title: Pathogenic variants in KCTD1 disrupt cAMP signaling and cellular communication associated with developmental pathways.
    Article Snippet: Our study utilized both male and female mice from the previously described cAMP Encoded Reporter (CAMPER) (C57BL/6Gt(ROSA)26Sortm1(CAG-ECFP*/Rapgef3/Venus*)Kama/J) (RRID:IMSR_JAX:032205) strain (36).

    Membrane:

    Article Title: A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium.
    Article Snippet: .. Of the following vectors the FP was exchanged for TqCa-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag, pLckmVenus-C1 (Addgene plasmid #84337) for a membrane tag, pmScarlet_Giantin_C1 (Addgene plasmid #85048) for a Golgi tag and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version. ..

    Article Title: A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium
    Article Snippet: .. Of the following vectors the FP was exchanged for Tq-Ca-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag, pLck-mVenus-C1 (Addgene plasmid #84337) for a membrane tag, pmScarlet_Giantin_C1 (Addgene plasmid #85048) for a Golgi tag and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version. ..

    Article Title: A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium
    Article Snippet: .. Of the following vectors the fluorescent protein was exchanged for Tq-Ca-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag, pLck-mVenus-C1 (Addgene plasmid #84337) for a membrane tag, pmScarlet_Giantin_C1 (Addgene plasmid #85048) for a Golgi tag and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version. ..

    Bioprocessing:

    Article Title: Pathogenic variants in KCTD1 disrupt cAMP signaling and cellular communication associated with developmental pathways
    Article Snippet: .. The following plasmids were obtained from Addgene: 3P-Luc (#113862), ICN1-GFP (#17623), Gli1 (#84922), NFAT-Fluc (#214665), mVenus-N1 (#addgene), 3xNLS-mTurquoise2 (#98817), pcDNA3.1 (#128034), AAV-hSyn-Cre-P2A-dTomato (#107738), AAV2/5 (#104964), and AdDeltaF6 (#112867). .. GαoA was obtained from the cDNA Resource Center (#GNA0OA0000).



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    FLIM images of HeLa cells co-transfected with <t>3xnls-Tq-Ca-FLITS</t> and G-Ca-FLITS-3xnes were acquired with a Leica Stellaris 8 before ( A ) and after addition of 100 µM histamine ( B ). False colors reflect the lifetime (average arrival time), according to the scale bar on the right. For the cyan channel, the excitation was at 440 nm and detection at 450–490nm and for the green channel, the excitation was at 490 nm and detection at 500–540nm.
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    FLIM images of HeLa cells co-transfected with <t>3xnls-Tq-Ca-FLITS</t> and G-Ca-FLITS-3xnes were acquired with a Leica Stellaris 8 before ( A ) and after addition of 100 µM histamine ( B ). False colors reflect the lifetime (average arrival time), according to the scale bar on the right. For the cyan channel, the excitation was at 440 nm and detection at 450–490nm and for the green channel, the excitation was at 490 nm and detection at 500–540nm.
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    Image Search Results


    FLIM images of HeLa cells co-transfected with 3xnls-Tq-Ca-FLITS and G-Ca-FLITS-3xnes were acquired with a Leica Stellaris 8 before ( A ) and after addition of 100 µM histamine ( B ). False colors reflect the lifetime (average arrival time), according to the scale bar on the right. For the cyan channel, the excitation was at 440 nm and detection at 450–490nm and for the green channel, the excitation was at 490 nm and detection at 500–540nm.

    Journal: eLife

    Article Title: A green lifetime biosensor for calcium that remains bright over its full dynamic range

    doi: 10.7554/eLife.105086

    Figure Lengend Snippet: FLIM images of HeLa cells co-transfected with 3xnls-Tq-Ca-FLITS and G-Ca-FLITS-3xnes were acquired with a Leica Stellaris 8 before ( A ) and after addition of 100 µM histamine ( B ). False colors reflect the lifetime (average arrival time), according to the scale bar on the right. For the cyan channel, the excitation was at 440 nm and detection at 450–490nm and for the green channel, the excitation was at 490 nm and detection at 500–540nm.

    Article Snippet: Of the following vectors, the FP was exchanged for Tq-Ca-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag, 4xmts-mScarlet-I (Addgene plasmid #98818), ER-mScarletI (Addgene plasmid #137805), and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version.

    Techniques: Transfection